In a study recently published in Neuron and featured in Nature Methods, the lab of Dr. Botond Roska performed targeted single-cell-initiated transsynaptic tracing to label retinal ganglion cells that provide input to individual principal cells in the mouse lateral geniculate nucleus (LGN).

Subsequently, more than 500 labeled ganglion cells were reconstructed using and PyKNOSSOS. An analysis of the morphology and innervation patterns of the individual ganglion cells revealed distinct modes of ganglion cell input integration by LGN principal cells.